Light responsive Gold NPs-polymer hybrid LBL capsules for the Lysosomal Storage Disorder

Authors

  • Meenakshi Gupta a Institute of Pharmacy, Chhatrapati Shahu Ji Maharaj University, Kanpur Uttar Pradesh-208024, India,b Department of Pharmaceutical Sciences, Faculty of Health Sciences, SHUATS Deemed University, Allahabad U.P.-211007
  • Prof.(Dr.) Sri Sivakumar Department of Chemical Engineering, Indian Institute of Technology Kanpur,d Centre for Environmental Science and Engineering, Indian Institute of Technology Kanpur, e Material Science programme, Indian Institute of Technology Kanpur,f Centre for Nanoscience and Soft Nanotechnology, Indian Institute of Technology, Kanpur,208016, India

Abstract

Abstract: GM1 gangliosidosis is a rare lysosomal storage disorder caused due to the malfunctioning of lysosomal ?-galactosidase that maintains the distribution of GM1 ganglioside within the body. Delivery of ? -galactosidase inside the cells is the only potential approach to cure such disease. However, trafficking of this therapeutic protein into a cell is highly challenging. In this regard, stimuli-responsive nanocarriers have gained a lot of attention as they precisely control the delivery of payload under the influence of a particular stimulus. To this end, we report the formulation of light-responsive gold NPs-polymer hybrid LBL capsule for the intracellular delivery of ?-gal enzyme and showed light-responsive breaking of gold NPs-polymer hybrid LBL capsule by using NIR laser exposure technique (980nm, a continuous laser beam of 1W power). The light-responsive breaking of gold NPs-polymer hybrid LBL capsules was verified by fluorescent microscopy and scanning electron microscope (SEM). We observed 1.5, 1.8- and 2.27-fold reduction in the capsules number after10 min, 15min and 20min laser exposure respectively. Further, ?-gal enzyme release from gold NPs-polymer hybrid LBL capsule was verified after capsule lysis using assay buffer (ONPG). We observed 1835mU±113 ?-gal entrapment within gold NPs-polymer hybrid LBL capsules (?500 nm). Gold NPs-polymer hybrid LBL capsules showed no cytotoxicity up to 150 capsules per cell concentration and were well internalized by the HeLa as well as L929 cells.

Keywords: Gangliosidosis, beta-galactosidase, gold nanoparticles, LBL capsules, light responsive, stimuli responsive nanocarriers

Author Biography

  • Prof.(Dr.) Sri Sivakumar, Department of Chemical Engineering, Indian Institute of Technology Kanpur,d Centre for Environmental Science and Engineering, Indian Institute of Technology Kanpur, e Material Science programme, Indian Institute of Technology Kanpur,f Centre for Nanoscience and Soft Nanotechnology, Indian Institute of Technology, Kanpur,208016, India

    Professor, Department of Chemical Engineering, Indian Institute of Technology Kanpur

Additional Files

Published

2021-06-14

Issue

Vol. 4 No. 1 (2021): International Journal of Community Science and Technology

Section

Article